
Diesel fuel quality testing is essential for maintaining engine performance and longevity. Poor-quality diesel can lead to inadequate combustion, reduced engine power, equipment damage, and increased emissions. To ensure optimal fuel performance and safety, it is crucial to regularly test diesel fuel for contaminants such as water, sediment, and microbial growth, as well as to assess combustion speed and stability and sulfur content. Untreated microbial contamination can lead to operational issues and fuel instability, resulting in significant costs for organizations that store or distribute large quantities of diesel fuel. Testing diesel fuel quality helps identify issues before they become problematic, ensuring safe and efficient fuel usage.
| Characteristics | Values |
|---|---|
| Contaminants | Water, debris, yeasts, molds, aerobic bacteria |
| Impact of contaminants | Blown injector tips, Filter plugging, fuel oxidation, damage to fuel injection systems |
| Fuel oxidation | Occurs in fuel over 30 days old, indicated by darkening of the fuel, leads to tank sludge |
| Test for fuel oxidation | Test #OX-20 |
| Test kit | Oxidation catalyst, five test vials, sampling pipettes |
| Cetane number | Assesses diesel fuel's ignition quality during compression |
| Cetane number range | 40-55 |
| High cetane levels | Make diesel engines difficult to start |
| Low cetane levels | Rough and noisy operation, higher instances of smoke at startup |
| Sulfur content | Significantly impacts emissions control, engine lubrication, and after-treatment systems |
| EPA limit on sulfur content | 15 ppm since 2007 |
| Density and distillation tests | Determine fuel composition, volatility, and suitability for specific climates |
| Microbial contamination | Occurs due to nourishment from fuel hydrocarbons and water |
| Impact of microbial contamination | Production of corrosive acids, damage to generators |
| Test for microbial contamination | 15-minute test used in over 130 countries |
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What You'll Learn

Testing for microbial contamination
Microbial contamination, also known as diesel bug or diesel algae, occurs when bacteria, yeasts, and fungi survive, grow, and negatively impact diesel fuel systems. Microbial contamination can cause serious operational issues and render the fuel unusable, leading to costly disruptions. Therefore, regular testing and monitoring are crucial to detect fuel contamination early and treat it inexpensively.
One commonly used test for microbial contamination is the Microbial Contamination test (FT-0110), which detects the level of microbial contamination in diesel and biodiesel fuels. This test screens for both fungal and bacterial contamination, which can cause issues such as filter plugging and fuel degradation if left untreated. The test is designed to be durable and can be performed in various environments, including remote locations where laboratory testing is impractical. It is recommended to conduct this test on a monthly basis for diesel and biodiesel storage tanks.
Another option for testing microbial contamination is the growth-based test, Liqui-Cult, which can be performed onsite or in a laboratory. This test takes 72 hours, as it involves observing visible growth within the fuel samples. The severity of contamination is determined by comparing the colour density of the sample with a provided colour chart. This test provides an indication of the level of microbial growth and helps identify potential issues before they become more severe.
To comply with regulatory requirements, such as the NFPA 110 standard, laboratory testing is often necessary. Facilities collect fuel samples from their tanks and ship them to specialised labs for analysis. These labs utilise prescribed ASTM test packages to measure various contaminants, including microbial contamination. By conducting these tests, organisations can ensure they meet regulatory standards and maintain fuel quality.
In addition to these tests, it is important to monitor and mitigate water contamination, as it is a primary contributor to microbial contamination. Water can enter fuel systems through condensation, rainwater, or adsorption from the air, creating favourable conditions for microbial growth. Water/Sediment testing can measure the amount of water and sediment suspended in the fuel, helping to identify potential issues early on.
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$27.04

Measuring the cetane number
The cetane number is a standard measure of the quality of diesel fuel's ignition during compression. It is a key indicator of the combustion speed and plays a similar role for diesel as the octane rating does for gasoline.
The cetane number is typically calculated by determining a blend of cetane and isocetane with the same ignition delay. Cetane has a cetane number of 100, while isocetane's measured cetane number is 15. Once the blend is known, the cetane number is calculated as a volume-weighted average, rounded to the nearest whole number, of cetane's 100 and isocetane's 15.
There are several methods to measure the cetane number. One traditional method is to use a special test engine called the Cooperative Fuel Research (CFR) engine. This method is considered the gold standard, but it is also time-consuming and costly. The operator of the CFR engine adjusts the compression and injection timing until the ignition performance matches that of reference fuels with known cetane numbers.
Another reliable method is the Ignition Quality Tester (IQT), which offers a simpler and more robust approach to CN measurement. Fuel is injected into a constant volume combustion chamber at approximately 575 °C and 310 psi (21 bar). The time between the start of injection and the recovery of the combustion chamber pressure to 310 psi is defined as the ignition delay, which is then used to calculate the derived cetane number (DCN) of the fuel.
Additionally, the Fuel Ignition Tester (FIT) is another instrument that provides a simpler alternative to the CFR engine test. Similar to the IQT, fuel is injected into a constant volume combustion chamber at a temperature of about 575 °C. The ignition delay is calculated as the time elapsed between the start of fuel injection and the start of combustion, which is indicated by a high rate of pressure change within the chamber. The fuel's derived cetane number can then be calculated using an empirical inverse relationship to ignition delay.
The cetane index (CI) is another statistic used to control fuel quality, which is based on the density and distillation range of the fuel. However, it is important to note that cetane index calculations cannot account for cetane improver additives and do not measure the total cetane number for additized diesel fuels.
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Assessing sulfur content
Assessing the sulfur content of diesel fuel is a critical aspect of fuel quality testing. High sulfur content in diesel fuel can have detrimental effects on engine performance, emissions, and the environment. Therefore, it is essential to conduct tests to ensure compliance with regulatory limits and maintain optimal fuel efficiency.
One widely recognised test method for determining sulfur content is the ASTM method D2622. This method employs wavelength dispersive X-ray fluorescence (WDXRF) to quantify sulfur concentration in petroleum products. WDXRF offers excellent sensitivity, straightforward sample preparation, and rapid data acquisition, making it well-suited for regulatory compliance. However, it is important to consider the sample matrix's effect on fluorescence yield, as variations in the concentrations of elements like carbon, hydrogen, and oxygen can impact the accuracy of sulfur measurements.
The European Union's 2009/30/EC directive sets a limit of 10 ppm of sulfur for diesel and gasoline. In North America, ultra-low-sulfur diesel (ULSD) standards mandate a maximum sulfur content of 15 mg/kg or 15 ppm. To meet these stringent requirements, fuel managers and business owners must regularly assess sulfur content to ensure safe and usable fuel.
Sulfur content in diesel fuel has a significant impact on emissions control, engine lubrication, and after-treatment systems. High sulfur content leads to the formation of sulfuric acid, causing corrosive wear on engine components. Additionally, sulfur compounds contribute to air pollution and catalyst toxicity, rendering after-treatment systems ineffective. Therefore, it is crucial to monitor and control sulfur levels to optimise engine performance and minimise environmental harm.
To address high sulfur content in diesel fuel, studies have suggested the use of gasoline-diesel blends and the implementation of the partially premixed combustion ignition (PPCI) concept. By blending gasoline with diesel and adjusting fuel injection timings, the overall sulfur content can be reduced, leading to improved engine performance and reduced exhaust emissions. This approach has been explored in countries like Iraq, where diesel fuel with high sulfur content is commonly used.
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Analysing water and sediment content
The ASTM D975 standard for diesel fuel quality encompasses seven grades and 13 tests, ensuring reliability and safety. One critical test is ASTM D2709, which specifically measures the presence of free water and sediment. This test employs centrifugation to isolate and quantify both free water and sediment particles in the fuel sample. Elevated levels of water and sediment indicate fuel instability, and remediation techniques such as fuel polishing and filtration are recommended.
To detect water intrusion, a simple and instant method is using Kolor Kut® Water Finding Paste, which changes colour upon contacting water. This paste is applied to a measuring stick inserted into the fuel tank, providing an indication of water depth. Additionally, test kits like the W-5 Test Kit from Dieselcraft offer a quick way to check for water in diesel fuel. These kits provide clear instructions and deliver results within seconds, helping identify potential issues before they cause engine damage.
For stored diesel fuel, regular monitoring of water levels in storage tanks is essential. Preventive measures, such as proper tank maintenance and spill prevention, limit water ingress. Fuel stabilisers are also recommended, especially for long-term storage, as they neutralise chemical reactions that lead to sediment formation.
In conclusion, analysing water and sediment content in diesel fuel is vital for maintaining fuel integrity and optimal performance. By employing testing methods, such as ASTM D2709, Kolor Kut® paste, and test kits, along with implementing preventive measures, fuel managers can ensure the reliability and longevity of their equipment.
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Testing for fuel oxidation
Fuel oxidation occurs when diesel fuel ages or undergoes microbial activity, increasing the fuel's acidity. This can cause issues with fuel injection systems over time. Testing for fuel oxidation helps identify potential issues before they arise, ensuring safe and efficient fuel usage.
One commonly used test for fuel oxidation is the #OX-20 test, which is simple, low-cost, and provides results in under 20 minutes. This test is designed to detect oxidation in diesel fuel that is over 30 days old, with the first sign of oxidation being darkening of the fuel. The test kit includes an oxidation catalyst, test vials, and sampling pipettes. If the fuel is oxidized, the test will indicate a black reaction layer in the test vial.
There are also other methods for testing fuel oxidation, such as the CEC L-105 and L-109 oxidation tests. These tests evaluate the resistance of oil formulations to biodiesel-induced engine oil oxidation. They involve treating fresh diesel engine oil with biodiesel in the presence of an iron catalyst to accelerate the reaction. The sample is then heated and aerated under controlled conditions, and viscosity increases are measured to determine the extent of oxidation.
Additionally, ASTM methods like RPVOT D2272, D943, and D4310 can be used to assess the oxidation stability of oils. These tests provide valuable data on the lifespan of lubricants and help prevent engine issues caused by lubricant degradation. Regular testing for fuel oxidation is essential for maintaining fuel quality and ensuring the optimal performance of diesel engines.
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Frequently asked questions
Untested diesel fuel can contain gasoline, which can lead to inadequate engine performance and equipment damage. Testing diesel fuel for gasoline helps to ensure complete combustion, maximising engine power and fuel efficiency.
Testing of diesel fuel should be done regularly to prevent issues with engine performance. Diesel fuel can start to oxidise after 30 days, so testing should be done at least this often.
A diesel fuel test will typically measure the cetane number, assess sulfur content, and analyse water and sediment content.
A small sample of the diesel fuel is distilled until 90% of it is recovered. The residue is then heated to 1022 degrees Fahrenheit, causing most of the sample to evaporate. The remaining residue is then weighed to measure carbon levels.
There are a variety of diesel fuel tests available, including on-site and laboratory tests. It is important to choose a testing method that reduces the risk of misleading results, as false positives can lead to unnecessary costs.











































