
The contamination of diesel fuel by microbes, including bacteria, is a common issue. This contamination, known as diesel bug, occurs when bacteria feed on the hydrocarbons in the fuel and create biomass, biofilms, and damaging by-products. The presence of water, either through condensation or rainwater, encourages microbial growth, as bacteria require tiny amounts of water and fuel to proliferate. This growth can lead to clogged filters, corrosion, and fuel degradation, resulting in operational issues and potential engine damage. Preventative measures, such as water separation and fuel tank insulation, are crucial to minimizing the risk of diesel bug.
| Characteristics | Values |
|---|---|
| Cause of bacteria growth | Water in the fuel tank |
| How water enters the tank | Condensation, rainwater penetration, adsorption from the air |
| Microbial growth locations | Interface between oil and water or on tank walls |
| Microbe types | Bacteria, fungi, yeast |
| Fuel types affected | Biodiesel, petro diesel |
| Fuel issues | Clogged filters, corrosion, higher oil consumption, fuel degradation |
| Prevention | Control water buildup, use biocides, tank insulation, routine tank cleaning and treatment |
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What You'll Learn

Water in diesel fuel
The presence of water in diesel fuel can result in rust particles entering the fuel and cause premature wear and corrosion in fuel injectors and other engine parts. Water can also reduce the fuel's energy content, leading to decreased engine power and hard starting, especially in cold weather. Water accumulation in fuel filters can lead to clogging and restricted fuel flow, and if left unaddressed, can result in costly repairs.
To prevent water contamination in diesel fuel, it is crucial to minimize the water level and manage the significant sources of water infiltration. Maintaining well-sealed fuel tank caps and regularly inspecting for leaks can help reduce the risk of water entering the fuel tank. Emphasizing proper fueling procedures and maintenance is essential for fleets and independent truckers to minimize human errors that contribute to water contamination.
Additionally, controlling tank water buildup is vital to inhibiting microbial growth. Microbes, including bacteria, thrive in the presence of water and can cause further issues such as filter blockage and increased corrosion due to their acidic by-products. Removing as much water content as possible from the diesel fuel is crucial to ensuring its purity and preventing issues associated with water contamination.
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Condensation in tanks
Condensation in diesel fuel tanks is a common issue, especially in large tanks that may take a long time to empty. The problem is that diesel is hygroscopic, meaning it absorbs water from the air. If you fill up your tank on a warm day, the diesel will absorb water until it reaches its saturation point. As the temperature drops, the diesel will no longer be able to hold all the water it has absorbed, and the excess will condense out as a separate liquid. This is made worse by the fact that diesel tanks are vented, meaning they are exposed to moist air, which will continually supply water to the fuel.
The issue with condensation in diesel tanks is that it provides an environment for microbial growth, which can cause significant problems for fuel systems. Microbes, including bacteria, fungi, and algae, are all around us and can enter fuel during transport and storage. They are microscopic and cannot be seen by the human eye, so even a tiny amount of water is enough for them to thrive. These microbes feed on the hydrocarbons in the fuel and create biomass, biofilms, and damaging by-products. They can coat surfaces and even 'climb' up storage tank walls, creating what is known as biofilm.
The best way to prevent microbial diesel fuel contamination is to control tank water buildup. This can be done through proper tank design, such as correctly sized and placed vents, and good housekeeping practices. However, even the best housekeeping methods can fail, especially if not everyone in the supply chain is doing the same thing. Additionally, it is virtually impossible to prevent all condensation in diesel tanks, especially if the tank is not kept at the same temperature as the surrounding air at all times.
Once microbial contamination occurs, it can be challenging to eradicate. The only way to permanently end it is to kill the microbes with a biocide. Controlling water is crucial, but as microbes don't need much water to grow and thrive, the chances are high that you'll have to deal with microbial contamination at some point. Regularly replacing fuel filters and injectors can help manage the issue, but it is a constant battle.
In summary, condensation in diesel fuel tanks is a significant issue as it provides an environment for microbial growth, which can cause extensive damage to fuel systems. While proper tank design and housekeeping practices can help reduce condensation and microbial growth, it is a constant challenge to manage and eradicate these issues.
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Microbial colonies
The presence of water is a critical factor in the growth of microbial colonies in diesel fuel. Water can enter diesel fuel through condensation, rainwater penetration, or adsorption from the air. Modern biodiesel, in particular, is highly hygroscopic, meaning it readily absorbs water from the surrounding environment. Even small amounts of water can provide the necessary conditions for microbial growth. The microbes thrive at the interface between the oil and water or on the tank walls, depending on their oxygen requirements.
Another factor contributing to microbial growth is the presence of a suitable food source, in this case, the fuel itself. Biodiesel, made from plant and animal fats, provides an especially good food source for microbes, leading to increased biological growth problems. However, microbes can also feed on petro diesel. Stagnant fuel that is left idle for long periods is particularly susceptible to microbial contamination.
Warm temperatures and the presence of biofuels accelerate the growth of microbial colonies. The ideal temperature range for microbial growth is between 10°C (50°F) and 40°C (104°F). Additionally, the condensation of water vapour within diesel fuel tanks creates favourable conditions for microbial proliferation and contamination.
The formation of microbial colonies can lead to several issues. They produce acidic by-products that increase corrosion and cause deposits that result in premature wear and tear on engine components. They also create biofilms, which can coat surfaces and block filters, injectors, and fuel systems. Over time, the fuel can degrade to the point of forming a slimy sludge that is unusable.
To prevent and control microbial growth in diesel fuel, it is essential to minimise the exposure of diesel fuel to water. This can be achieved through procedures such as fuel recycling, water separation, and the routine discharge of water from fuel tanks. Additionally, fuel tank insulation can help stabilise fuel temperature and inhibit microbial growth. Regular tank inspections, cleaning, and treatments with approved diesel fuel biocides are also recommended to control and eliminate microbial contamination.
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Biodiesel as food for microbes
Biodiesel is an eco-friendly, renewable, and sustainable alternative to fossil fuels. It is derived from vegetable oils, non-edible oils, waste oils, and microbial oils. The use of microbial oils for biodiesel production has gained attention due to its sustainability and environmental benefits.
Microbial contamination is a significant issue in diesel fuel, commonly known as "diesel bug." Diesel bug refers to the growth of microorganisms, including bacteria, fungi, and yeast, in diesel fuel. These microbes thrive in the presence of water, which can enter diesel fuel through condensation, rainwater, or adsorption from the air. Modern biodiesel, in particular, tends to attract water due to its hygroscopic nature.
The microbes feed on the hydrocarbons in the fuel, leading to the creation of biomass and biofilms, and harmful by-products. They can grow at the interface between oil and water or on the tank walls, depending on their oxygen requirements. The microbial contamination results in clogged filters, corrosion, and damage to fuel systems.
To address this issue, controlling water buildup in storage tanks is crucial. However, even with proper housekeeping, microbial contamination may still occur due to the small amounts of water needed for microbial growth. In some cases, biocides may be necessary to eradicate the problem.
While biodiesel itself can be susceptible to microbial contamination, certain types of microbes are also being explored as feedstock for biodiesel production. Oleaginous bacteria, for example, have a high growth rate, are easy to cultivate, and can utilize various substrates for growth. They can metabolize different carbohydrates and waste organic materials, making them a potential sustainable and cost-effective feedstock for biodiesel production.
In conclusion, while microbes can indeed contaminate diesel fuel, the focus on microbial oils as a feedstock for biodiesel production offers an eco-friendly and sustainable alternative to fossil fuels. The advantages of using oleaginous bacteria as feedstock include their high growth rate, ease of cultivation, and ability to utilize various substrates. However, the economic viability of microbial oils for biodiesel production is still uncertain due to the high cultivation costs.
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Testing and prevention
Testing for microbial contamination in diesel fuel can be done using test kits such as FUELSTAT® and FuelTurbo Microbial test kits. FUELSTAT® uses an immunoassay antibody test that provides rapid screening of fuel samples. It measures the severity of microbial contamination and provides actions and alert levels within minutes. The FuelTurbo Microbial test kit uses a unique paddle formulation that supports and identifies the growth of common bacteria and micro bug cells found in diesel fuels. The test results may appear in as little as 24 hours or less.
To use the FuelTurbo Microbial test kit, follow these steps:
- Obtain a sample of diesel, preferably from the bottom of the fuel tank, the fuel filter, or the water separator.
- Immerse the test paddle into the diesel sample for about 10 seconds, ensuring that all the test gel is coated.
- Allow excess diesel to drip off the paddle and remove any remaining drips with a paper or cloth towel.
- Place the slide back into the housing and store it upright at a temperature between 25°C and 35°C (77°F to 95°F).
- For bacterial detection (cream side of the paddle), incubate for 24 to 36 hours.
- For yeast, mould, and bug detection (brown side of the paddle), incubate for 36 to 48 hours.
- Compare the results to the provided charts to interpret the presence and severity of microbial contamination.
To prevent microbial contamination in diesel fuel, the key is to control water buildup and practise good fuel husbandry. Water can enter diesel fuel through condensation, rainwater penetration, or adsorption from the air, and it provides an ideal environment for microbes to grow. Regularly remove water from fuel tanks and control condensation, especially in hot and humid conditions. Additionally, consider using products specifically formulated to control water buildup, such as Bell Performance Dee-Zol and DFS Plus.
In some cases, the use of biocides may be necessary to control microbial contamination. Bell Performance Bellicide is a biocide that can effectively kill microbes. However, in severe cases, complete cleaning of the tanks, fuel remediation, or even a total fuel write-off may be required, which can be extremely costly. Regular testing and proactive water management are the most cost-effective ways to prevent and control diesel bug problems.
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Frequently asked questions
Bacteria grow in diesel fuel when there is water present, creating the right conditions for microbial growth.
Bacteria need water, a suitable temperature range (10°C to 40°C), and a food source (in this case, fuel) to grow and thrive.
Bacterial growth in diesel fuel can lead to the formation of acid, rust, corrosion, and clogged filters. It can also result in the degradation of fuel quality and cause operational issues.
To prevent bacterial growth, it is crucial to minimise the exposure of diesel fuel to water. This can be achieved through water separation, routine discharge of water bottoms, fuel tank insulation, and regular tank cleaning and inspections.











































