
Diesel fuel contamination by microbes, often referred to as diesel algae, is a common issue for diesel users. Despite its name, it is not actually algae, as fuel tanks are too dark for algae to grow. Instead, it is a sludge-like substance composed of microbes such as bacteria, mould, and fungus. This substance can cause significant issues by clogging filters and damaging engines. Therefore, it is important to regularly test for microbial contamination in diesel fuel and take preventative measures such as using fuel additives or automated fuel maintenance systems to avoid costly repairs.
Characteristics and Values of Testing for Algae in Diesel Fuel
| Characteristics | Values |
|---|---|
| Testing methods | Microbe tests, ATP tests, culture tests, automated fuel maintenance systems, mobile fuel polishing systems |
| Test results | Positive: presence of algae/microbes; Negative: no algae/microbes detected |
| Symptoms of algae/microbe presence | Clogged filters, decreased engine efficiency, sludge buildup, fuel degradation, potential damage to storage tanks, inaccurate fuel gauge readings |
| Causes | Water presence in diesel fuel, condensation, old diesel, microbial contamination |
| Prevention | Use fuel additives, biocides, fuel maintenance systems, regularly drain water, maintain fuel system cleanliness |
| Treatment | Remove water, clean tank, use fuel polishing systems, biocides, or chemicals |
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What You'll Learn

Testing for algae with microbe tests
When considering diesel fuel contamination by microbes, it is important to be vigilant. Microbial contamination can compromise fuel performance and equipment integrity. For generators and diesel storage tanks, fungus, mould, and bacteria pose a serious threat as they can clog fuel filters and decrease efficiency.
There are different kinds of in-field microbe tests available. The best kind to use is an ATP test. Culture tests are generally not recommended for this kind of application. One such test involves dipping a test strip into the fuel and waiting a day. If there is no reaction, the test is negative for algae. If there is a reaction, the test is positive for algae. This type of test uses a specialized media to detect microorganisms in diesel fuel.
Another test for detecting algae is the ASTM G29 test. This test is used to evaluate the efficacy of antimicrobial-treated surfaces against algae growth. It is applicable for polymeric films used in man-made water structure environments such as swimming pools, irrigation ditches, water storage tanks, and artificial ponds. The test typically takes 14-30 days, with the results determined by visually inspecting the samples for algae growth.
A third option for testing for algae is the "jar test". This test is used to assess water quality and can be performed by obtaining a sample of water from a pond or lake and placing it in a clear glass or plastic jar. The jar is then left to sit overnight, after which it can be inspected for signs of algae growth. If the jar is very dark or has scum on the top, it could indicate the presence of dissolved organic acids or cyanobacteria, respectively.
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Using fuel polishing systems
While algae cannot grow inside a diesel fuel tank due to the absence of sunlight, microbial growth, often referred to as diesel "algae", can occur. This sludge-like substance can wreak havoc on your fuel filters and engine, leading to reduced engine power and expensive repairs. To prevent and address this issue, fuel polishing systems can be employed.
Fuel polishing systems work by circulating fuel out of the tank for filtration, removing both large and small contaminants, including water, sediment, and bacteria. This process helps to prevent future microbial contamination and maintain fuel quality. It is important to note that a single fuel polishing service may not prevent microbial growth indefinitely, and multiple servicings throughout the year may be necessary. Additionally, fuel additives that remove water from the fuel can be used in conjunction with fuel polishing to further inhibit microbial growth.
The FuelTurbo Mobile Diesel Fuel Polishing System is an example of a professional and cost-effective solution for diesel fuel and tank contamination. It can be used to clean, recondition, renew, and polish contaminated diesel fuel and tank systems in a variety of applications, including marine vessels, power generators, and automotive vehicles. The system is low maintenance and can be operated by anyone with basic mechanical skills, making it a versatile and user-friendly option.
Implementing a fuel polishing system can be an effective strategy for removing algae and other contaminants from diesel fuel. However, it is also important to take preventive measures, such as regularly cleaning and maintaining the fuel tank and fuel lines, to inhibit the growth of microbes and maintain the health of your diesel fuel system.
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Using biocides to kill microbes
The presence of microbes in diesel fuel can lead to several issues, including fuel degradation, filter clogging, corrosion, and injector damage. To address these problems, biocides can be used to kill the microbes and prevent further growth.
Biocides are effective in killing microbes such as bacteria and fungi that contribute to fuel contamination. When using a biocide, it is important to follow certain steps to ensure its effectiveness. Firstly, add the biocide to the fuel in the tank, and then fill the tank with more fuel to achieve the recommended concentration of the biocide. This ensures that the biocide is properly diluted and can effectively target the microbes.
Additionally, consider using a biomass dispersant chemical alongside the biocide. Biomass, or "biofilms," created by microbes can stick to solid surfaces and protect them from the biocide. By using a dispersant, the biomass formations can be broken up, allowing the biocide to penetrate and kill a higher number of microbes.
Circulation of the fuel is critical to the success of the biocide treatment. Proper circulation ensures that the biocide is completely blended and reaches all areas where microbes may be present. However, it is important to note that even with effective biocides, dead microbial bodies can still settle at the bottom of the tank and plug fuel filters. Therefore, regular filter replacements may be necessary during the treatment process.
While biocides are useful in killing microbes, they do not address the root cause of fuel contamination, which is often the presence of water in the fuel tank. In fact, repeated use of biocides may eventually become ineffective as microbes can develop resistance over time. As an alternative, fuel filtration equipment can mechanically remove solid contaminants and water, eliminating the environment that allows microbes to proliferate.
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Removing water to prevent algae
While algae is a microscopic plant organism that requires sunlight to grow, what is commonly referred to as "diesel algae" is, in fact, a type of bacteria or fungus that grows in the water-diesel fuel interface, with water being an essential requirement for its growth. Therefore, removing water from diesel fuel is a crucial step in preventing the growth of these microbes.
Regularly Drain Water from the Tank
Use a tank stick to measure the amount of water accumulated at the bottom of your diesel tank. If the water level exceeds half an inch, it is recommended to mechanically drain the water. You can do this by removing the filter screen at the bottom of the tank and either cleaning or replacing it. This is an important first step in remediating any microbial contamination.
Use Water-Absorbing Chemical Treatments
After draining the water, it is essential to follow up with a water-absorbing chemical treatment to eliminate any remaining water droplets or residues. While this step is crucial, it may not entirely solve the problem, as it does not directly kill the microbes.
Treat with a Diesel Additive
The use of a diesel additive is recommended to remove water from the fuel. These additives can help break down the water-fuel interface, making it inhospitable for the growth of microbes.
Maintain and Clean the Fuel Tank
Regular maintenance and cleaning of the fuel tank and fuel lines are essential to prevent the buildup of debris, sediment, and biomass produced by microbial activity. Proper care can help inhibit the growth of microbes and maintain the health of your diesel tank.
Use High-Quality Diesel Fuel
Opting for high-quality diesel fuel can reduce the likelihood of contamination by microbes. Lower-quality diesel may contain more impurities and contaminants that can serve as a food source for microbial growth.
Use a Fuel Stabilizer
If you anticipate that your engine will be inactive for extended periods, consider using a fuel stabilizer. Fuel stabilizers can help prevent the growth of bacteria and other microbial contamination by inhibiting their growth.
Remember, while removing water is essential, a comprehensive approach that targets multiple factors contributing to microbial growth will be most effective in preventing algae in diesel fuel.
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Signs of algae: clogged filters, black specks
While algae is not the correct term for the microbial contamination of diesel fuel, it is commonly used to refer to the presence of mould, fungus, or bacteria in the fuel. This contamination can cause serious issues for diesel generators and storage tanks, as it can lead to clogged fuel filters and decreased efficiency.
One of the key signs of algae in diesel fuel is an abnormal increase in the rate at which filters become clogged. The microbial bodies, along with the black, slimy biomass matrix they produce, can plug the filters. Additionally, the activity of the microbes can cause the fuel to break down faster, further contributing to filter clogging. If you notice that you are going through filters at a faster rate than usual, it is recommended to check your tank for microbes.
Another sign of algae is the presence of small black specks in the clear pre-filters. If you notice these black specks, it is important to inspect the inside of your fuel tank. Removing the fuel sending unit will allow you to examine the tank for signs of algae growth. If the inside of the tank appears black, it is likely that you have algae growth.
To confirm the presence of algae, you can perform a microbe test. An ATP test is recommended over a culture test for this purpose. While dealing with algae in diesel fuel can be challenging, understanding the signs of contamination and taking prompt action can help prevent further issues and maintain the efficiency of your diesel systems.
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Frequently asked questions
If you notice small black specks in your clear pre-filters, you may have algae in your diesel fuel. You can also inspect the inside of your fuel tank by removing the fuel sending unit. If the inside of your tank is black, you likely have algae.
Algae in diesel fuel can cause your filters to clog and your engine to malfunction or become damaged. You may also notice a decrease in fuel efficiency.
Algae, or microbes, grow in the water-diesel fuel interface. Water is the food source for algae, and it can enter your fuel tank through condensation.
There are in-field microbe tests available, such as ATP tests, that can detect the presence of algae or microbes in diesel fuel. These tests are dipped into the fuel and will react within 24 to 36 hours if microbes are present.
To prevent algae growth, you can use automated fuel maintenance systems, fuel additives, biocides, or mobile fuel polishing systems. Maintaining a clean fuel system and removing water from the fuel are also important for preventing algae growth.











































