
Diesel fuel contamination by microbes, often referred to as algae, is a common issue that can wreak havoc on fuel filters and engines. This contamination is caused by bacteria, fungi, and other microbes that thrive in the water-diesel interface, leading to the formation of a dark sludge that clogs filters and affects fuel quality. While it is not truly algae, as algae require light to grow, the presence of these microbes can have detrimental effects on fuel systems. To prevent and address diesel algae, it is crucial to regularly test fuel quality, detect contamination early, and implement proactive measures such as maintaining optimal fuel storage conditions, including temperature and water accumulation. Various testing kits are available to identify even small amounts of algae, allowing for timely intervention and targeted strategies to eradicate the issue.
Characteristics and Values of Testing Diesel Fuel for Algae
| Characteristics | Values |
|---|---|
| Testing methods | Use a testing kit, visual inspection, ATP test, culture test |
| Testing kit characteristics | User-friendly, can identify small amounts of algae, require training to interpret results |
| Visual inspection | Check for clogged filters, reduced engine power, sludge |
| Prevention | Maintain fuel storage temperatures below algae growth threshold, minimise airspace in fuel tanks, use older fuel first, ensure adequate tank ventilation, schedule periodic professional cleaning, regularly test fuel quality, choose reputable fuel suppliers |
| Treatment | Remove water from the tank, use biocides to kill microbes, circulate fuel after adding biocide, allow time for dead microbes to settle, use extra fuel filters |
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What You'll Learn

Testing kits: Use a kit to detect algae/microbes
Testing kits are a reliable way to detect algae or microbial contamination in diesel fuel. These kits are designed to monitor microbial contamination, which can lead to sludge buildup, fuel degradation, and potential damage to storage tanks and engines.
One such kit is the Combination DS-1 Test Kit plus FCT-100 Fuel Clarity and Water Test offered by Dieselcraft. This kit can be used to detect algae and bacteria in diesel fuel and is suitable for use in the USA, Canada, and Australia.
Another option is the FT-BUG Test Kit, which can detect bacteria, bugs, algae, and fungus in diesel fuel. This kit uses a unique paddle formulation, with one side coated in a TTC Agar gel with red spot technology, and the other side coated with a laboratory-formulated gel. The paddle is simply inserted into the fuel sample or swiped on the fuel sample, and the results can appear in as little as 24 hours or even less. This kit is also available with a water detector or as part of a combo test kit.
To use the FT-BUG Test Kit, simply remove the test paddle from the housing and immerse it into a sample of diesel fuel for 10 seconds, ensuring that all the test gel is coated. It is important not to touch or contaminate the slide gel strips. The sample can be taken from the bottom of the fuel tank, the fuel filter, the water separator, or directly from the tank into a suitable container.
These testing kits are an excellent way to monitor the amount of microorganisms present and take preventive action before more severe problems occur.
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Visual inspection: Check for signs of algae like clogged filters
While algae cannot grow inside a diesel fuel tank due to the lack of sunlight, microbes like mould, bacteria, and fungi can contaminate the fuel. These microbes can wreak havoc on your fuel filters and engine, so it's important to regularly inspect your fuel for signs of contamination.
Visual inspection is a good starting point if you suspect an infestation based on symptoms like clogged filters or reduced engine power. Keep an eye on your fuel filters and track how often you need to replace them. If you notice an abnormal increase in filter plugging rate, it could be a warning sign of microbial contamination. The microbes themselves, as well as the black, slimy biomass they produce, can clog the filters. Additionally, the presence of microbes can cause the fuel to break down faster, further contributing to filter clogging.
If you suspect microbial contamination, you can visually inspect the inside of your fuel tank. Remove the fuel sending unit and check for signs of contamination. If you notice small black specks in your clear pre-filters or if the inside of your tank is black, you may have algae growth or microbial contamination.
It's important to note that visual inspection may not always be possible, and you may need to rely on other methods, such as microbe tests, to confirm the presence of contamination. However, visual inspection can be a simple and effective way to initially assess the condition of your fuel and identify potential issues.
If you visually confirm the presence of algae or microbes, it is recommended to take appropriate action, such as removing the water from the tank, cleaning the tank, and replacing contaminated filters. Additionally, consider using fuel additives or biocides to prevent future contamination and maintain the quality of your diesel fuel.
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Water presence: Algae grows in water, so check for water
Water and diesel fuel are a bad combination. While small amounts of water in diesel fuel are common, excessive amounts can cause major issues. Water in diesel fuel can promote the growth of algae and bacteria, which can lead to corrosion and damage to the steel tank and other engine components. Therefore, it is important to regularly check your diesel fuel for water to prevent these issues.
There are several ways to check for water in your diesel fuel. One method is to use a water in diesel testing kit, which can quickly and safely indicate the presence of water in your fuel. These kits are designed to detect water in diesel fuel specifically and are commonly used in industries that require clean fuel systems, such as automotive, marine, and aviation.
Another way to check for water is to visually inspect your diesel tanks. Clean diesel fuel should appear clear and light-coloured. If the fuel looks dark or cloudy, it may indicate the presence of water in the system. Additionally, you can use a flashlight to look into the tanks and observe the colour of the fuel.
Most diesel-powered vehicles are equipped with a water separator in the fuel system. It is important to regularly check and drain this separator to ensure it is not gathering large amounts of water. This simple maintenance task can help prevent water contamination in your diesel fuel.
Finally, you can also observe the rate at which you go through fuel filters. Diesel fuel algae can plug filters rapidly due to the microbial bodies and the black, slimy biomass matrix they produce. An abnormal increase in filter plugging can be a warning sign of water contamination and the presence of algae or bacteria in your diesel fuel.
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Biocides: Use chemicals to kill microbes
Biocides are a popular method to combat microbial contamination in diesel fuel. They are effective in killing microbes such as bacteria and fungi, which can cause issues such as fuel degradation, filter clogging, corrosion, and injector damage. The presence of microbes in diesel fuel can be identified by mucous-like accumulations on fuel filters and the need to replace them more frequently.
To use a biocide effectively, it is important to follow certain steps. Firstly, add the biocide to the fuel in the tank, ensuring that the final concentration of the biocide meets the recommended level. For instance, Bellicide suggests using one ounce of biocide for every 40 gallons of fuel. Secondly, fill the tank with more fuel to reach the desired capacity. This ensures that the biocide is adequately mixed and circulated throughout the fuel, maximizing its effectiveness in killing microbes.
Additionally, consider using a biomass dispersant chemical simultaneously with the biocide. This helps to break down and disperse the biomass formations created by microbes, allowing the biocide to penetrate and kill a higher number of microbes. However, it is important to note that even with the use of biocides, dead microbial bodies can still settle at the bottom of the tank and plug fuel filters. Therefore, it is recommended to change the filters regularly to prevent any issues.
While biocides are effective in killing microbes, they do not address the root cause of the problem, which is the presence of water in the fuel tank. Water serves as a breeding ground for microbes, and by removing it, the proliferation of microbes can be prevented. In this regard, fuel filtration equipment offers a superior alternative to biocides by mechanically removing water and solid contaminants from diesel fuel, eliminating the root cause of microbial growth.
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Prevention: Keep fuel tanks dry and well-maintained
Keeping fuel tanks dry and well-maintained is key to preventing the growth of diesel "algae". While it is not actually algae (as algae are microscopic plant organisms that require light to grow, and fuel tanks are too dark), the microbes that grow in diesel fuel are often referred to as diesel "algae". These microbes include bacteria, mould, and fungus, which can wreak havoc on your fuel system, causing clogged filters, engine damage, and performance issues.
To prevent the growth of these microbes, it is important to eliminate their ideal growth environment. This environment is created by three main components: air, water, and temperature. By removing any one of these components, you can prevent microbial growth. Here are some specific ways to do this:
- Keep your fuel tanks full to reduce air space and minimise condensation.
- Minimise the airspace in fuel tanks to reduce condensation and water accumulation.
- Use older fuel first to prevent prolonged storage, which can increase the risk of microbial growth.
- Ensure adequate tank ventilation to prevent moisture buildup and maintain optimal environmental conditions.
- Regularly clean and maintain your fuel tank and fuel lines to prevent the buildup of debris, sediment, and organic matter.
- Treat your fuel with a diesel additive that removes water from the fuel, and frequently drain the water from your water separator.
- Use high-quality diesel fuel that is less likely to contain contaminants and impurities.
- If your engine will be sitting for a while, consider using a fuel stabilizer to prevent the growth of bacteria and other microbes.
By implementing these proactive measures, you can effectively prevent the growth of diesel "algae" and maintain the health of your fuel system.
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Frequently asked questions
If you notice small black specks in your clear pre-filters, this could be a sign of algae growth. You can also inspect inside your fuel tank by removing the fuel sending unit. If it is black inside, you probably have algae.
Algae is a misnomer as it is not actually algae but rather a combination of species of bacteria, moulds, fungi, and other microbes. These microbes need water to grow and thrive. Water can form in your fuel tank through condensation.
There are testing kits available that can identify even small amounts of algae. These kits are designed to be user-friendly and can be used by those without extensive technical knowledge.
You should remove the filter screen in the bottom of the tank and clean or replace it. You should also drain any water accumulated at the bottom of your tank. You can then use a dedicated biocide to kill the active microbial contamination.











































