
Diesel bug, or microbial contamination, is a common issue for diesel users. It occurs when bacteria, moulds, and fungi grow in diesel fuel, leading to engine damage and system failure. This contamination can be prevented by using a fuel stabilizer or biocide treatment, such as Bio Kleen Diesel Fuel Biocide, which kills microbes and prevents fuel filter plugging. Regular testing and maintenance are crucial to avoid costly repairs and ensure the optimal performance of diesel engines. Proactive measures, such as using high-quality diesel fuel and fuel additives, can also help prevent diesel bug. Additionally, anti-bacterial diesel additives can be used to prevent the formation of fungi and bacteria, serving as a cost-effective solution.
Characteristics and Values Table for Killing Bacteria in Diesel Fuel
| Characteristics | Values |
|---|---|
| Cause of Bacteria in Diesel Fuel | Water getting into diesel fuel due to condensation, rainwater or air adsorption |
| Microbe Types | Bacteria, filamentous moulds, yeasts, fungi |
| Fuel Type Prone to Microbial Growth | Ultra Low Sulfur Diesel (ULSD) |
| Symptoms of Microbial Contamination | Mucous-like accumulations on fuel filters, increased fuel-filter replacement, corrosion, clogged injectors and filters |
| Prevention | Use fuel stabilizers, anti-bacterial additives, biocides, periodic kill doses, efficient filter systems |
| Testing | FUELSTAT, Adenosine Triphosphate (ATP) test, CFU tests |
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What You'll Learn

Use a biocide treatment to kill bacteria
Biocide treatments are a commonly used method to get rid of bacteria in diesel fuel. Diesel biocide is a chemical additive that kills bacteria in diesel fuel on contact. It works by penetrating the cell walls of the bacteria, causing them to rupture and die. This prevents the bacteria from growing and multiplying, reducing the risk of fuel contamination.
When using a biocide treatment, it is important to follow the manufacturer's instructions to ensure safety and effectiveness. It is also crucial to add enough biocide to the fuel to achieve the recommended concentration. For example, Bellicide recommends a concentration of 1 ounce per 40 gallons to shock and kill an existing microbial contamination. Additionally, circulation of the fuel is critical to ensure that the biocide comes into contact with the bacteria. Mixing a dispersant chemical with the biocide can also help break up biomass formations, allowing the biocide to penetrate and kill more microbes.
It is worth noting that biocides are toxic and can harm more than just the bacteria in the fuel. Overusing biocides can lead to the development of biocide-resistant strains of microbes. Therefore, proper care and maintenance of the fuel system are important to prevent microbial growth. This includes restricting the infiltration of water, using high-quality diesel fuel, and adding fuel stabilizers or antioxidants.
Overall, using a biocide treatment is an effective way to kill bacteria in diesel fuel and prevent fuel contamination. However, it should be used as part of a comprehensive fuel maintenance plan to avoid potential drawbacks and ensure the longevity of the diesel engine.
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$38.89

Prevent water infiltration to limit bacteria growth
Water is an essential element for microbes to establish themselves and multiply, as they live in the water-diesel interface. Therefore, preventing water infiltration is key to limiting bacteria growth.
Firstly, it is important to understand how water can enter a diesel fuel system. Water can infiltrate a diesel fuel system through leaky storage tanks, poor or degraded tank seals, or human error, such as leaving a fuel tank cap off. Additionally, diesel fuel has hygroscopic qualities, meaning it attracts and absorbs moisture from the air at the molecular level, so it almost always contains some water.
To prevent water infiltration, it is recommended to buy diesel fuel from reputable sources with well-maintained systems, as most fuel retailers and distributors use water-detecting filters in their dispensing equipment to prevent water from entering the fuel system. However, these filters must be adequately maintained, as they can become saturated and allow water to be dispensed with the fuel.
Another way to prevent water infiltration is to keep the fuel tank full, as this reduces the amount of air in the tank and helps prevent condensation. Regular maintenance of the fuel system is also crucial, including inspecting the fuel tank for leaks, replacing filters regularly, and draining water from the fuel system.
Using diesel fuel additives can also help prevent water contamination. These additives work by breaking down water molecules, allowing them to be burned off by the engine. Some additives also contain anti-bacterial agents that can prevent bacterial growth.
By implementing these measures, water infiltration can be limited, which is essential to restricting bacteria growth in diesel fuel systems.
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Test for bacteria with a fuel contamination kit
Microbiological contamination in diesel fuel is a common problem, caused by bacteria, yeasts, and fungi that negatively impact diesel fuel systems. Water entering the fuel system is a common cause of bacterial growth, and condensation is a key source. Testing for contamination is important, as early detection can prevent mechanical issues and fuel degradation.
There are several fuel contamination kits available that can detect bacteria. One such kit is the FT-BUG Test Kit, which uses a unique paddle formulation. One side of the paddle is covered in a gel that identifies the growth of common bacteria and micro bug cells found in diesel fuels. The other side of the paddle is coated in a gel that identifies the growth of fungi, yeast, mold, and algae cells. To use the kit, you must first remove the test paddle from the housing. Then, immerse the test paddle into the diesel sample for 10 seconds, ensuring that all the test gel is coated. Results may appear in as little as 24 hours or less. If the test indicates problems, you should consider treating your fuel with a biocide. This kit does not require any special equipment or training and can be safely disposed of after use.
Another option is the FUELSTAT® Diesel Plus – FMD8 Fuel Test Kit, which is an immunoassay antibody test that detects all relevant microbes in just 15 minutes. This test is simple to run, requires no laboratory or scientific expertise, and can be performed on-site.
The Microbial Contamination test (FT-0110) is another option that detects both fungal and bacterial contamination. This test does not yield immediate results, as it takes time to measure the contamination level effectively. However, it is durable and can be performed in most environments.
Finally, CFU (colony-forming units) and ATP (adenosine triphosphate) tests are also available to detect microbial contamination in diesel fuel. CFU tests use a fuel sample to grow any culturable microorganisms present, under suitable conditions. ATP tests involve quantifying the total ATP present in the sample by measuring the emitted light.
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Use anti-bacterial diesel additives
One of the most common issues with diesel fuel is microbial growth, often called diesel "algae". This sludge-like substance is caused by microbes, including bacteria, that feed on the hydrocarbons in the diesel fuel and produce waste products that cause corrosion and engine fouling. These microbes will live happily in even the smallest amounts of water, often from condensation, and at the interface of fuel and water, where they can create thick mats known as biomass.
To prevent and treat diesel algae, you can use anti-bacterial diesel additives, also known as biocides. These additives are designed to kill bacteria and other contaminants in diesel fuel. They are commonly used to treat microbial contamination in diesel fuel and are effective in both diesel fuel and water.
When choosing an anti-bacterial diesel additive, look for a product that is compatible with your diesel fuel type and read the label carefully to determine the correct dosage. For example, the LIQUI MOLY anti-bacterial diesel additive is compatible with all conventional diesel and biodiesel fuels, while the Bio Kleen Diesel Fuel Biocide Treatment is effective in both diesel fuel and water.
To use an anti-bacterial diesel additive, follow the instructions on the product label. As a general guide, for preventative use, add the corresponding amount of additive before filling the tank. For serious bacterial and fungal infestations with filter clogging, use a booster dose. It is recommended to add the additive directly to the fuel tank and then drive the vehicle to help the additive pass through the entire system, protecting all components.
In addition to using anti-bacterial diesel additives, there are other measures you can take to prevent and treat diesel algae. Proper care and maintenance of your diesel fuel system are crucial. Keep the fuel system clean and use high-quality diesel fuel and fuel additives. Regularly test your fuel to detect any microbial contamination early on, as it can cause significant damage to your engine and fuel system if left untreated.
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Keep diesel tanks clean and well-maintained to prevent engine damage
Diesel bug, or microbial contamination, is a common issue for diesel users. It refers to the growth of microbes, including bacteria, filamentous moulds, yeasts, and fungi, in diesel fuel. These microbes feed on the hydrocarbons in the fuel and create waste products that can cause corrosion and engine damage. To prevent engine damage, it is important to keep diesel tanks clean and well-maintained.
One way to maintain diesel tank health is to use a fuel stabilizer or an anti-bacterial diesel additive. These products can help prevent the growth of bacteria and other microbial contamination. Additionally, proper care and maintenance of the diesel system are crucial. This includes regularly checking for signs of contamination and testing the fuel. Testing kits, such as FUELSTAT®, can quickly and accurately assess the presence and severity of microbial contamination.
Another method to keep diesel tanks clean is to use a mobile fuel polishing system. This technology circulates fuel out of the tank, filters it, and then returns it to the tank. It is also important to address the presence of water in diesel fuel systems, as water provides a breeding ground for microbes. Removing water from diesel tanks can significantly reduce the risk of microbial contamination.
In some cases, a periodic kill dose of biocide may be necessary to treat microbial contamination. However, it is important to use biocides with caution, as they can be toxic and may lead to the development of biocide-resistant microbes. Taking proactive measures, such as using high-quality diesel fuel and fuel additives, can help prevent the need for extensive treatments. Regular maintenance and cleaning of diesel tanks can also help prevent the growth of microbes and maintain the health of the fuel system.
By following these measures, diesel tank users can effectively prevent microbial contamination and reduce the risk of engine damage. Proper diesel tank maintenance includes a combination of preventative measures, regular testing and cleaning, and the appropriate use of additives and treatments when needed.
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Frequently asked questions
Diesel bug is a contamination of diesel fuel by microbes such as bacteria and fungi. Water can get into diesel fuel through condensation, rainwater, or adsorption from the air, encouraging microbial growth.
You can use a test kit like FUELSTAT® to detect the presence of microbes in your diesel fuel. Alternatively, look out for symptoms such as corrosion, clogged filters, and engine deposits.
To prevent diesel bug, you should proactively keep your fuel system clean and use high-quality diesel fuel and fuel additives. You can also use a fuel stabilizer or anti-bacterial diesel additive to prevent the growth of bacteria.
To get rid of diesel bug, you can use a biocide treatment to kill the microbes. However, be careful not to use biocide treatments too often, as this can lead to strains of microbes that are biocide-resistant.











































